The purpose of this study is to investigate the implication of start-up ambidexterity toward competitive advantage by building an integrated model that combines the dynamic capability and ambidexterity concept through organizational learning, innovation, and change. There were 100 startup founders/co-founders that participated in the survey. The Hierarchical component Model (HCM) was established using Partial Least Square – Structural Equation Model (PLS-SEM) to model the 100 start-up founders/co-founders’ quantitative data gathered from the regional events using the proposed model. There are three main results from this study, the first result shows that the start-up ambidexterity has a positive and significant effect on competitive advantage, second results show at this stage of startup development, they have more exploration behavior compared with the exploitation, and lastly, that innovation capability is more dominant capability owned by these startups. This study contributes to integrating several disciplines using the proposed integrated model on start-up survivability by understanding through testing, their behavior, and capability, especially in the early-stage organization development.